# ![@axionlabs Avatar](https://lunarcrush.com/gi/w:26/cr:youtube::UC15cOaD0N6Edidyft1KwvRw.png) @axionlabs Axion Labs & Training Institute

Axion Labs & Training Institute posts on YouTube about axion, how to, gas, lee the most. They currently have [-----] followers and [--] posts still getting attention that total [---] engagements in the last [--] hours.

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- [--] Week [-----] +0.93%
- [--] Month [-----] +4.80%
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### Social Influence

**Social category influence**
[cryptocurrencies](/list/cryptocurrencies)  100% [stocks](/list/stocks)  2.08%

**Social topic influence**
[axion](/topic/axion) 93.75%, [how to](/topic/how-to) 33.33%, [gas](/topic/gas) 18.75%, [lee](/topic/lee) 18.75%, [the most](/topic/the-most) 8.33%, [virtual](/topic/virtual) 6.25%, [check out](/topic/check-out) 6.25%, [what is](/topic/what-is) 6.25%, [topics](/topic/topics) 6.25%, [flow](/topic/flow) 6.25%

**Top assets mentioned**
[Axion (AXN)](/topic/axion) [Waters Corporation (WAT)](/topic/waters-corporation) [Helium (HNT)](/topic/helium)
### Top Social Posts
Top posts by engagements in the last [--] hours

"Demystifying GC Method Development - When to Change Your Column 👉 Get the Chromatography Demystified Mini-course with a free Axion Lite membership. https://courses.axionlabs.com/course/axion-lite/#/sales Separating xylene isomers is a challenging real-world problem in gas chromatography. In this video Dr. Lee Polite walks step by step through GC method development using a classic xylene mixture: ortho- meta- para-xylene and ethylbenzene. Youll see what happens when a standard scouting run produces co-elution why temperature and flow optimization alone are sometimes not enough and how"  
[YouTube Link](https://youtube.com/watch?v=Z3GBs_QvAOo)  2026-02-10T16:10Z [----] followers, [---] engagements


"GC Troubleshooting - The most common problems you will encounter. Check out our first online virtual course: https://axionlabs.com/lc-gc-bootcamp-virtual-course/ Chapters: 0:00 What is wrong with my GC - an overview 0:30 Half splitting technique 1:17 #1 a leaky septum 2:25 #2 the liner 3:41 GC troubleshooting at Axion Labs How do I know if my septum is leaking When do I change my liner How can I learn GC troubleshooting Dr. Polite gives some quick tips to help you with your GC troubleshooting"  
[YouTube Link](https://youtube.com/watch?v=WMa-oIR18S8)  2021-04-19T13:23Z [----] followers, [----] engagements


"12 most commonly asked HPLC and GC interview questions with answers. Get the extended version of this video plus download the Interview Prep Guide. https://axionlabs.com/hplc-gc-interview-guide/ Are you preparing for an HPLC or GC job interview Get a massive advantage with this expert guide In this video Dr. Lee Polite a seasoned chromatography expert reveals the [--] most commonly asked HPLC and GC interview questions and provides the practical insightful answers that hiring managers are truly looking for. Don't let lack of experience hold you back. Prepare smarter interview confidently and"  
[YouTube Link](https://youtube.com/watch?v=gYhSMAuw3c0)  2025-06-17T13:10Z [----] followers, [----] engagements


"What's the difference between HPLC & UPLC Let me give you the technical difference between HPLC & UPLC. UPLC first of all is a registered trademark of Waters Corporation. Other companies call their version of UPLC something like UHPLC or STM or something other than UPLC. Read the blog here: https://axionlabs.com/chromatography-training/uplc-and-hplc-whats-the-difference-video/ 🔗 Follow Us: Website: https://axionlabs.com/ Facebook: https://www.facebook.com/axionlabs LinkedIn: https://www.linkedin.com/company/axion-labs/ #Chromatography #HPLC #ScientificResearch #analyticalchemistry #chemistry"  
[YouTube Link](https://youtube.com/watch?v=ai5IMwrTh2o)  2023-10-17T16:00Z [----] followers, [----] engagements


"Importance of Innovation in Gas Chromatography Agilent introduced their revolutionary new type of GC on 8/30/2016 at their headquarters in Little Falls Delaware: the Agilent [----] Intuvo. Six years ago Dr. Polite was one of only [--] thought leaders from around the world called in to help Agilent with the design criteria. Lee was often referred to as the voice of the customer during the design process. The goal was to make the GC easier to use while not sacrificing any of the chromatographic performance. It has been [--] years in the making and the result is pretty impressive. No more column nuts"  
[YouTube Link](https://youtube.com/watch?v=feV-lkxnbVY)  2016-08-30T21:22Z [----] followers, [---] engagements


"Demystifying GC Method Development 👉 Get the Chromatography Demystified Mini course with a free Axion Lite membership. https://courses.axionlabs.com/course/axion-lite/#/sales In this in-depth session Dr. Lee Polite walks shows how to develop and optimize a gas chromatography (GC) method from the ground up. He starts with a simple universal scouting run using a standard non-polar column (DB-5 type) and a wide temperature program. From there Dr. Polite demonstrates how to systematically optimize for speed and efficiency cutting run times dramatically by adjusting linear velocity (flow rate)"  
[YouTube Link](https://youtube.com/watch?v=lpHul-21A_8)  2026-01-27T20:36Z [----] followers, [---] engagements


"How to develop a method for GC - top [--] tips How do you develop a method for GC Ill give you three quick trips for GC method development. 00:11 Intro and Analogy 00:58 #1 Look for a method in a column catalog 01:28 #2 Choose a good standard column 02:15 #3 Try all the temperatures in the universe 📌 Want more chromatography tips Join our free community to receive HPLC and GC training from an expert. https://courses.axionlabs.com/course/axion-lite/#/home GC method development sounds daunting But the reality is that it is a lot simpler than it sounds. Trick #1: Look in a column catalog. I know"  
[YouTube Link](https://youtube.com/watch?v=0pRfAEMeBls)  2021-07-29T21:16Z [----] followers, [----] engagements


"Understand and Fix Negative Peaks - Refractive Index (RI) Detectors 🔬 This video dives into the mystery of negative peaks specifically with refractive index (RI) detectors Learn why they happen (it's not an error) and how to easily integrate them using your software. We also touch on unexpected negative peaks in UV detection and what they might mean. Read the blog article: https://axionlabs.com/chromatography-training/negative-peaks-in-hplc-refractive-index-and-uv-detectors/ 🚀 Stop scratching your head and master your HPLC data 📌 Want more chromatography tips Join our free community to"  
[YouTube Link](https://youtube.com/watch?v=n3b8nx-vXi8)  2025-05-10T14:15Z [----] followers, [----] engagements


"Why is the GC beeping Why is the gas chromatograph beeping How do I Fix it Learn how to troubleshoot a beeping Gas Chromatograph (GC) Discover why the instrument might be beeping and how to check its status for error messages like "no hydrogen gas" or "issue getting the flame lit". See how adjusting the pressure can make your GC "happy" and stop the beeping 📺 Related Video: GC Operations Explained Full System Breakdown https://youtu.be/LaYPRxOuu54 🎓 Want to go deeper Get a free Axion Lite membership: https://courses.axionlabs.com/course/axion-lite#/salesticketId=3283437000000185030 🌐 Learn"  
[YouTube Link](https://youtube.com/watch?v=-BFmPk6NU6M)  2025-06-05T19:45Z [----] followers, [---] engagements


"HPLC Diode array parameters and tools - REFERENCE WAVELENGTH PEAK PURITY and THRESHOLD 💡 Should the Spectra Range Include the Reference Wavelength Let's break it down In this video we explore reference wavelengths in chromatography why they matter and whether they should be included in spectral data. Plus we dive into peak purity analysis and how to detect coelution using ChemStation. 🚀 🔹 Topics Covered: ✔ What happens when reference wavelength is activated ✔ How to prevent negative peaks ✔ Understanding diode array detector (DAD) signal processing ✔ Peak purity analysis and how to detect"  
[YouTube Link](https://youtube.com/watch?v=-sDL3KTPN9E)  2025-03-18T20:06Z [----] followers, [---] engagements


"LC/GC Bootcamp - Master HPLC & GC in one week at Axion Training Institute's Check out our in person and virtual courses: https://axionlabs.com/lc-gc-bootcamp/ https://axionlabs.com/advanced-hplc/ https://axionlabs.com/advanced-gc/ https://axionlabs.com/lc-gc-bootcamp-virtual-course/ Learn HPLC and GC at Axion Training Institutes 5-day LC/GC Bootcamp. Get hands-on applied training in our chromatography training center in Chicago. Get course dates and more information at https://axionlabs.com/lc-gc-bootcamp/"  
[YouTube Link](https://youtube.com/watch?v=6D9bZy3JtSk)  2023-10-20T20:45Z [----] followers, [----] engagements


"How to install an HPLC column"  
[YouTube Link](https://youtube.com/watch?v=6Th7P2IZXCI)  2022-10-05T14:51Z [----] followers, [----] engagements


"Top [--] tips for HPLC method development Check out our in person and virtual courses: https://axionlabs.com/lc-gc-bootcamp-virtual-course/ https://axionlabs.com/lc-gc-bootcamp/ https://axionlabs.com/advanced-hplc/ https://axionlabs.com/advanced-gc/ https://axionlabs.com/lc-gc-bootcamp-virtual-course/ Develop HPLC methods with two tips from Axion Labs: [--]. Don't reinvent the wheel - check out working methods in an HPLC column catalog. [--]. Perform a scouting run. Check out https://axionlabs.com/ for upcoming course dates"  
[YouTube Link](https://youtube.com/watch?v=6wLdOefZioI)  2021-04-05T12:37Z [----] followers, 13.1K engagements


"Can acetonitrile cause amine impurities to form deposits in HPLC pump Have you ever wondered if acetonitrile in HPLC can cause amine impurities or lead to sticky deposits in your pump In this video we explore whether acetonitrile polymerization is a real issue and how to prevent potential HPLC check valve problems. 🔬 Key Topics Covered: ✅ Does acetonitrile contain amine impurities ✅ Can acetonitrile deposits damage HPLC pumps ✅ Are certain HPLC systems more affected ✅ Best solvents to flush your HPLC system. ✅ The real issue: Buffer precipitation vs. polymerization. 💡 Pro Tips for HPLC"  
[YouTube Link](https://youtube.com/watch?v=7oIXdZ_-8H0)  2025-02-03T17:14Z [----] followers, [---] engagements


"HPLC Demystified Part [--] Chromatography Theory Explained With Kool-Aid 🎁Download the PDF lesson guide https://zfrmz.com/fjn7iuq3bsK0mnFJyqFu Ever struggle to understand how separation works in HPLC You're not alone. In this video Dr. Lee Polite breaks down the chemistry behind chromatography using the worlds most approachable demo: grape Kool-Aid. Timestamps 00:00 Intro 00:37 How does HPLC chromatography work 00:52 Shopping mall analogy 05:38 About the Kool Aid experiment 07:47 Polarity explained 09:13 Start of experiment Learn the key concepts that make liquid chromatography work including:"  
[YouTube Link](https://youtube.com/watch?v=9Ns2nhiuNHA)  2025-07-07T11:53Z [----] followers, [----] engagements


"3 Essential Gas Generators for Gas Chromatography Explained: Hydrogen Air & Nitrogen Generators 🚨 Upgrade Your GC Lab: Why Smart GC Labs Use These [--] Gas Generators Looking to ditch your gas cylinders and streamline your gas chromatography (GC) workflow In this video we break down the [--] essential gas generators every GC lab should consider: ✅ Hydrogen Generator For carrier gas & FID fuel ✅ Zero Air Generator For hydrocarbon-free oxidant gas ✅ Nitrogen Generator For make-up gas & detector efficiency 🔬 We explain how each generator improves lab safety efficiency and cost savings and why so"  
[YouTube Link](https://youtube.com/watch?v=Bt1-hXtvc9o)  2025-05-17T03:15Z [----] followers, [---] engagements


"black friday sale (720 x [---] px).mp4 Be ready for [----] with serious HPLC and GC skills. Get 25% off the Dec. 9-13 session of the hands-on LC/GC Bootcamp at Axion Labs. https://zurl.co/cadj"  
[YouTube Link](https://youtube.com/watch?v=DFMqeuWfMsw)  2024-11-25T17:04Z [----] followers, [---] engagements


"HPLC Demystified Part [--] How to Maintain and Troubleshoot Your HPLC Like a Pro 🎁Download our HPLC Maintenance & Troubleshooting Checklist https://zfrmz.com/fjn7iuq3bsK0mnFJyqFu 🔧 HPLC Demystified Part 3: How to Maintain and Troubleshoot Your HPLC Like a Pro Chromatography giving you headaches Part [--] of the HPLC Demystified series is your lab-side guide to troubleshooting and routine maintenance explained clearly by Dr. Lee Polite. Whether you're dealing with air bubbles leaky seals noisy baselines or a worn-out rotor seal this video walks you through the why the how and the fix. 00:00 Intro"  
[YouTube Link](https://youtube.com/watch?v=FfLIjBVGpeQ)  2025-08-28T11:43Z [----] followers, [----] engagements


"Tour of Axion Labs I've been teaching chromatography for over [--] years and I've learned that the learning environment is just as important as what I know and can teach. That's why I am so proud of Axion Labs. I've been working for over [--] years to make it the best learning environment so that you can master HPLC and GC. Check out our 5-day hands-on LC/GC Bootcamp schedule for [----] here: https://axionlabs.com/lc-gc-bootcamp/ 0:00 Chromatography Happy Place - the HPLC's and GC's dedicated to training 0:40 Where the magic happens - aha moments galore in the classroom 0:59 What you need to bring"  
[YouTube Link](https://youtube.com/watch?v=Jjd1Ur3kYXU)  2022-11-03T17:52Z [----] followers, [---] engagements


"HPLC Demystified Part [--] How an HPLC System Works. 🎁Download the PDF lesson guide https://zfrmz.com/fjn7iuq3bsK0mnFJyqFu Think HPLC systems are complicated Not after this. In Part [--] of the HPLC Demystified series Dr. Lee Polite opens the hood of a real instrument and walks you through each component in a way that finally makes sense. Whether youre prepping for a job troubleshooting in the lab or just want to stop feeling intimidated by your LC system this video is for you. 00:00 Intro 00:28 Mobile Phase (Solvent Bottles) 02:53 Degasser 05:44 Pro Tip About The Degasser 07:06 Pump 13:55"  
[YouTube Link](https://youtube.com/watch?v=Nw9CGJgSJf8)  2025-07-16T12:49Z [----] followers, [----] engagements


"Co-Elution: How to Detect and Fix Overlapping Peaks. Co-elution is the silent killer of chromatography datatwo compounds exit the column at the same time creating one misleading peak. That means you can't identify quantify or trust your results. In this video Dr. Lee Polite explains what causes co-elution how to detect it using peak shape DAD and Mas Spec and how to fix it using the resolution equation: capacity factor selectivity and efficiency. Perfect for anyone working with HPLC or GC especially if you're troubleshooting poor resolution or planning method development. 📘 Explore hands-on"  
[YouTube Link](https://youtube.com/watch?v=QkEVi6BJKuM)  2025-07-03T14:20Z [----] followers, [----] engagements


"How to print an HPLC calibration curve How to print an HPLC calibration curve Go to File then Print and then navigate over to Calibration Table and Curves. If you click that it's a beautiful printout. This question came from this video - https://youtu.be/p1qN0szuqjcsi=EpQEIfeAbVCV-EbH 📌 Want more chromatography tips Join our free community to receive HPLC and GC training from an expert. https://courses.axionlabs.com/course/axion-lite/#/home 📌 Got more HPLC questions Drop them in the comments 🔔 Subscribe for more chromatography insights #HPLC #Chromatography #LabScience #HPLCAnalysis"  
[YouTube Link](https://youtube.com/watch?v=WHNj2PcJmOY)  2025-03-11T20:45Z [----] followers, [---] engagements


"What is the difference between running green stainless vs red stainless HPLC tubing at 0.3mL/min Are you running your HPLC system at [---] mL/min and wondering whether to use green or red stainless steel tubing In this video we break down the key differences between these two tubing options and explain how choosing the right one can improve efficiency and peak resolution in low-flow applications like LC-MS. 💡 Key Topics Covered: ✅ Why green tubing (0.17 mm ID) is standard for most flow rates ✅ When to switch to red tubing (0.12 mm ID) for low-flow applications ✅ How tubing diameter affects"  
[YouTube Link](https://youtube.com/watch?v=WOOOAT2qNBw)  2025-02-11T15:23Z [----] followers, [---] engagements


"Understanding The Master Resolution Equation In Chromatography Unlock the secrets to perfect peak separation in your chromatographic methods In this comprehensive guide we break down the Master Resolution Equation a fundamental concept in chromatography (liquid chromatography gas chromatography) that once understood can significantly simplify your method development and troubleshooting efforts. Stop the guesswork and start making informed decisions Learn how the three critical factors Efficiency (N) Selectivity () and Capacity Factor (k) directly impact your separation quality. We explain"  
[YouTube Link](https://youtube.com/watch?v=WiApAwaDcsw)  2025-05-22T16:10Z [----] followers, [---] engagements


"Welcome to Axion Labs & Training Institute What can you expect from Axion's HPLC & GC training courses Rookie to Pro Experienced to Expert After completing Axion's HPLC & GC training courses you will have advanced HPLC and GC skills no matter your starting point. Learn more at https://axionlabs.com/ #chromatography #analyticalchemistry #scienceexplained #chemistry #chemistrytips #hplc #hplccolumns #labtechniques"  
[YouTube Link](https://youtube.com/watch?v=X79S_zx7ENY)  2022-08-10T11:59Z [----] followers, [----] engagements


"Superficially Porous vs Totally Porous HPLC Columns Higher Efficiency Lower Pressure Whats the difference between superficially porous (SPP) and totally porous (TPP) particles in HPLC columns In this video we explain how column particle structure affects efficiency separation and pressure requirements. Youll learn: Why smaller HPLC particles increase efficiency but demand higher pressure How superficially porous particles (core-shell) give you UPLC-like efficiency on standard HPLC systems The pros and cons of SPS vs TPS columns for real-world chromatography applications Whether youre running"  
[YouTube Link](https://youtube.com/watch?v=XafsUxmi77Q)  2025-08-19T20:08Z [----] followers, [---] engagements


"How does mass spec work Watch the full webinar on Axion Lite - Free HPLC and GC training. https://courses.axionlabs.com/course/axion-litepreviouspage=allcourses&isenrolled=no#/salesticketId=3283437000000185030 Subscribe for more insights into chromatography techniques and applications. 🔗 Follow Us: Website: https://axionlabs.com/ Facebook: https://www.facebook.com/axionlabs LinkedIn: https://www.linkedin.com/company/axion-labs/ #analyticalchemistry #hplc #Chromatography #LabTechniques #ScientificResearch #Chemistry #labtips Axion offers hands-on HPLC (High-performance liquid chromatography"  
[YouTube Link](https://youtube.com/watch?v=f1Z-4-tRDCM)  2024-05-14T18:22Z [----] followers, [---] engagements


"How to convert your gas chromatograph from helium to hydrogen - Axion Labs Webinar Check out our first online virtual course: https://axionlabs.com/lc-gc-bootcamp-virtual-course/ Chapters 0:00 Why hydrogen for my GC 2:54 Axion Training Institute 6:06 What is gas chromatography 10:27 GC Theory basics 17:04 GC Instrumentation overview 18:41 Switch to hydrogen in one step 22:21 Example chromatograms 23:31 Safety considerations 26:06 A real GC run with helium 28:31 Switching to hydrogen 31:53 Tour of Axion Labs 34:38 A real GC run with hydrogen 37:33 Method optimization with hydrogen 41:40"  
[YouTube Link](https://youtube.com/watch?v=jST8GDgUrrk)  2021-04-13T17:16Z [----] followers, [----] engagements


"Pyrolysis GC: How to Analyze Paint Plastics and Polymers with Gas Chromatography. 🔗 Resources Mentioned Watch the full GC operations video: https://youtu.be/LaYPRxOuu54si=Lmh18rfrW-g8R5Gb 🎓 Want to go deeper Visit Axion Lite for on-demand training in GC and HPLC: https://courses.axionlabs.com/course/axion-lite#/salesticketId=3283437000000185030 🔥 What Is Pyrolysis GC and Why Does It Matter In this video we break down one of the most fascinating techniques in gas chromatographyPyrolysis GC. Discover how scientists and forensic experts use this method to analyze plastics paints and polymers"  
[YouTube Link](https://youtube.com/watch?v=k5_qZGxn_vk)  2025-04-22T14:37Z [----] followers, [---] engagements


"When to use a gradient in HPLC How do you know when you should use an gradient elution instead of isocratic elution In this exploration of gradients in chromatography we unpack the pivotal question: when is a gradient necessary The heart of the matter lies in the hydrophobic similarities of the molecules being analyzed. If they share similar hydrophobicities a gradient might not be needed as indicated by a scouting run where peaks emerge closely together. However for a diverse range of hydrophobicities or unknown samples gradients shine by offering versatility and the invaluable ability to"  
[YouTube Link](https://youtube.com/watch?v=p9tVn4nJItc)  2024-03-19T15:24Z [----] followers, [----] engagements


"Will highly acidic or basic samples affect an HPLC C18 column Should you worry about the pH of a sample damaging the column What happens when your sample isnt in the same pH as the mobile phase I break it all down in simple terms So this is in response to a video I did about the use of guard columns. https://axionlabs.com/chromatography-training/understanding-hplc-guard-columns-necessity-and-best-practices/ The general rule in HPLC is that we always want to dissolve the sample in the mobile phasethat is the ideal situation. If the sample solvent is the same as the mobile phase it will just"  
[YouTube Link](https://youtube.com/watch?v=qJu9QXhyDTA)  2025-03-04T19:29Z [----] followers, [---] engagements


"HPLC Columns Explained Understanding Carbon Load and Surface Area for Better Separations Join our free community to get on-demand courses: https://courses.axionlabs.com/course/axion-lite/#/sales Why does one C18 column behave differently from another The answer often comes down to two overlooked details: carbon load and surface area. In this video Dr. Lee Polite breaks down: How carbon load is measured in HPLC columns Typical carbon load ranges (7% to 30%) and what they mean Why higher or lower carbon load changes retention and capacity How surface area (100300 m/g) impacts column performance"  
[YouTube Link](https://youtube.com/watch?v=qzPN0fDorVc)  2025-09-03T12:22Z [----] followers, [---] engagements


"How to fix "GC-MS vial out of range" error -🔧 GC-MS Error: Vial Out of Range Quick Fix Explained Running into a "vial out of range" error on your Agilent GC or GCMS system Dont worryits a common mistake with an easy fix. In this video we walk through what causes this error and how to solve it especially if you're using an Agilent [----] autosampler. 👉 Want more FREE training on GC and HPLC Check out Axion Lite our free online platform with videos tips and expert training: https://courses.axionlabs.com/course/axion-lite/#/sales 📌 Topics Covered: -Mass Spec [--] part series -HPLC Method"  
[YouTube Link](https://youtube.com/watch?v=tCXzsWA98-M)  2025-04-02T16:01Z [----] followers, [---] engagements


"Agilent Lab Advisor https://www.agilent.com/en/product/liquid-chromatography/hplc-ce-software/instrument-control-software/lab-advisor-software"  
[YouTube Link](https://youtube.com/watch?v=5uiQnEQhMo0)  2022-09-01T15:04Z [----] followers, [----] engagements


"How to choose the right HPLC column - AND - favorite HPLC columns from Agilent Waters & Phenomenex How do I choose the right HPLC column So Step [--] - look in a column catalog. Its gonna give you a guaranteed result. OK let's say that you don't have a column catalog. Or lets say that your chemical isnt in one. Lets say that you are sitting there saying Well Lee if I knew all of that I wouldnt be asking you for advice. So that is a valid point. Let me take you to the next step. In HPLC we have dozens of columns out there but there is one column that dominates. If you look at all the column sales"  
[YouTube Link](https://youtube.com/watch?v=9j8hWVY2neY)  2021-08-27T19:22Z [----] followers, [----] engagements


"Air bubble in the pump. The simple fix #chromatography #hplc @axionlabs"  
[YouTube Link](https://youtube.com/watch?v=AsT4ubvAl9o)  2023-03-28T13:13Z [----] followers, 16K engagements


"In gas chromatography what is column flow purge flow split ratio 💡 Understanding Column Flow Purge Flow and Split Ratio in Gas Chromatography (GC) 💡 In this video we break down the key concepts of column flow purge flow and split ratio in GC: ✅ Column Flow The carrier gas moving through the column ✅ Purge Flow A small stream (3 mL/min) that removes contaminants from the septum ✅ Split Flow The portion of the sample that is vented out instead of entering the column ✅ Split Ratio The ratio of split flow to column flow (e.g. 100:1 means 1% of the sample enters the column) Read the blog here:"  
[YouTube Link](https://youtube.com/watch?v=DjiY8IXfNAw)  2025-02-17T16:25Z [----] followers, [----] engagements


"How are HPLC and GC used in the pharmaceutical industry Read the blog here: https://axionlabs.com/chromatography-training/chromatography-applications-in-pharmaceuticals/ The pharmaceutical industry is huge in chromatography because in that industry they must by law analyze their raw materials to prove that theres nothing bad in it. Pharmaceutical companies are always looking for top professionals with valuable skills. Axion offers hands-on HPLC (High-performance liquid chromatography or high-pressure liquid chromatography) and GC (gas chromatography) training courses. Gain advanced HPLC & GC"  
[YouTube Link](https://youtube.com/watch?v=EFl2doSqvm4)  2021-04-19T13:36Z [----] followers, 11.4K engagements


"GC Sample Prep Essentials: Nailing Solvents Dilution & Injection 👉 Want to learn more about running the instrument Watch our video on GC Operations: https://youtu.be/LaYPRxOuu54 👉 More in-depth training and free videos at Axon Lite: https://courses.axionlabs.com/course/axion-lite/#/sales Struggling to get consistent reliable results from your Gas Chromatograph Proper sample preparation is the critical first step and in this video we break down the absolute essentials for chemists. Learn why sample volatility is non-negotiable in GC analysis and how anything non-volatile gets left behind in"  
[YouTube Link](https://youtube.com/watch?v=GeaAbGZjOcI)  2025-04-18T19:00Z [----] followers, [---] engagements


"Clean a reverse phase HPLC Column in [--] steps. Cleaning a reverse-phase HPLC column doesnt have to be complicated. In this step-by-step guide Dr. Lee Polite explains how to flush out salts buffers and sticky non-polar residues using simple solvent changes. Youll learn exactly when to use water-rich mobile phase when to switch to 100% organic and how to keep your columns running longer. Prefer to read: https://axionlabs.com/chromatography-training/clean-reverse-phase-hplc-column/ 👉 Related Videos: How to Clean a C18 Column extra details on cleaning a dirty or clogged frit"  
[YouTube Link](https://youtube.com/watch?v=HPCIeCyGlYk)  2025-09-10T22:19Z [----] followers, [----] engagements


"Gas Chromatography Demystified - Understanding How A GC Works 👉 Want on-demand training on GC and HPLC Check out Axion Lite our free online platform with videos tips and expert training: https://courses.axionlabs.com/course/axion-lite/#/sales Feeling intimidated by Gas Chromatography (GC) 🤔 Think it's too complex with all those buttons gases and parts This video breaks down GC operation into easy-to-understand steps Timestamps 00:00 Video overview 01:12 Understanding gases in GC helium cylinders 04:04 Understanding gas generators nitrogen hydrogen zero air 09:00 Understanding how gases flow"  
[YouTube Link](https://youtube.com/watch?v=LaYPRxOuu54)  2025-04-17T19:37Z [----] followers, 12.5K engagements


"HPLC Method Tips for Aromatic Compounds in Water Based Gels: Why C18 Still Works Learn how to analyze aromatic compounds in water-based gels using HPLC and why a C18 column is still one of the most reliable choices. In this clip Dr. Lee Polite breaks down how water interacts with a C18 stationary phase why gel matrices arent a problem in reverse-phase chromatography and how to set up a simple gradient method to get clean dependable separations. If youre working with gels creams lotions or other complex water-based materials this video will help you choose the right column solvent system and"  
[YouTube Link](https://youtube.com/watch?v=MTQcAwr7fr4)  2025-11-18T19:29Z [----] followers, [---] engagements


"HPLC Troubleshooting Reproducibility Tips HPLC Reproducibility Problems Its NOT Your Method. Heres What to Check First. Getting different chromatograms from the same HPLC sample is one of the most frustrating problems in the lab. Different peak areas. Different heights. Sometimes even a different number of peaks. In this short video Dr. Lee Polite explains why this is not a method problem and how to quickly diagnose reproducibility issues in your HPLC system without rewriting your method. Youll learn: -Why the same vial should always produce the same chromatogram -What extra or missing peaks"  
[YouTube Link](https://youtube.com/watch?v=S1fgKBu5Slk)  2026-01-08T16:56Z [----] followers, [---] engagements


"It doesn't matter which brand GC or HPLC you use. Agilent Waters Shimadzu PerkinElmer Does it matter which one I use. NO I'm a non-denominational instructor. In other words a GC is a GC. Read the blog here: https://axionlabs.com/chromatography-training/does-it-matter-which-hplc-or-gc-brand-i-use/ 🔗 Follow Us: Website: https://axionlabs.com/ Facebook: https://www.facebook.com/axionlabs LinkedIn: https://www.linkedin.com/company/axion-labs/ #analyticalchemistry #hplc #Chromatography #LabTechniques #ScientificResearch #Chemistry #labtips Axion offers hands-on HPLC (High-performance liquid"  
[YouTube Link](https://youtube.com/watch?v=UX13422-d14)  2022-12-05T20:50Z [----] followers, [---] engagements


"Why Your GC Inlet Total Flow Drops During a Temperature Ramp And How to Fix It Why does your GC inlet total flow drop during an oven temperature ramp In this video Dr. Lee Polite explains how gas viscosity changes during heating why constant pressure causes flow decline and why switching to constant flow mode gives you better more stable chromatography. Watch the video GC Troubleshooting - The most common problems you will encounter. : 👉 https://www.youtube.com/watchv=WMa-oIR18S8 Learn more about GC & HPLC at Axion Labs: https://axionlabs.com #gaschromatography #gctroubleshooting"  
[YouTube Link](https://youtube.com/watch?v=X05Rvi-5hrU)  2025-12-08T16:23Z [----] followers, [---] engagements


"Why My HPLC Columns Last [--] Years: The Truth About pH and Column Damage. Unlock the real secret to HPLC column longevity. Most scientists think column failure comes from dirty samples or too many injections but thats not the real culprit. In this video Dr. Lee Polite explains why pH is the single most important factor that determines whether your column lasts [--] injections or [--] years. Youll learn: Why sample pH almost never matters. The exact pH range every silica-based column loves. What happens chemically above pH [--] and below pH [--]. When it's safe to cheat at extreme pH. Which specialty"  
[YouTube Link](https://youtube.com/watch?v=XxiUdday3p8)  2025-11-25T19:38Z [----] followers, [---] engagements


"How to test potency of THC GC or HPLC 🧪 How to Test THC Potency GC vs. HPLC Are you wondering which instrument is best for testing THC potency in cannabisGas Chromatography (GC) or High-Performance Liquid Chromatography (HPLC) In this video we break down the key differences. 00:00 🔬 How THC potency is measured 01:14 📊 HPLC vs. GC Key differences 02:00 💡 Use HPLC for differentiating THC & THCA 02:05 🔥 GC is best for total THC measurement Read the blog here: https://axionlabs.com/chromatography-training/how-to-test-thc-potency-gc-or-hplc/ Want more tips about sample prep Watch the webinar"  
[YouTube Link](https://youtube.com/watch?v=qWf_2cmOJt0)  2025-02-25T17:31Z [----] followers, [----] engagements


"What's unique about Axion courses See what an Axion course is like. Subscribe for more insights into chromatography techniques and applications. 🔗 Follow Us: Website: https://axionlabs.com/ Facebook: https://www.facebook.com/axionlabs LinkedIn: https://www.linkedin.com/company/axion-labs/ #analyticalchemistry #hplc #Chromatography #LabTechniques #ScientificResearch #Chemistry #labtips Axion offers hands-on HPLC (High-performance liquid chromatography or high-pressure liquid chromatography) and GC (gas chromatography) training courses. Gain advanced HPLC & GC skills with our [--] day hands-on"  
[YouTube Link](https://youtube.com/watch?v=xAqymcjjDsg)  2024-04-01T19:57Z [----] followers, [----] engagements

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