@axionlabs Axion Labs & Training Institute

Axion Labs & Training Institute posts on YouTube about axion, how to, gas, lee the most. They currently have [-----] followers and [---] posts still getting attention that total [---] engagements in the last [--] hours.

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Social Influence

Social category influence cryptocurrencies social networks technology brands stocks

Social topic influence axion #35, how to, gas, lee, flow, high performance, whats the, what is, what s, the most

Top assets mentioned Axion (AXN) Waters Corporation (WAT) Helium (HNT)

Top Social Posts

Top posts by engagements in the last [--] hours

"HPLC Tips: How to calculate void volume In this video we delve into the critical aspect of calculating void volume in High Performance Liquid Chromatography (HPLC). Understanding the void volume or the space within the column not occupied by the stationary phase is essential for precise chromatographic analysis. We start by introducing T0 measurementhow to determine the time at which solutes pass through the column without interacting with the stationary phase. Using uracil as a common example we demonstrate practical methods for visually identifying T0 through disturbances or sine waves on"
YouTube Link 2024-02-06T19:16Z [----] followers, [----] engagements

"HPLC Demystified Part [--] Chromatography Theory Explained With Kool-Aid 🎁Download the PDF lesson guide https://zfrmz.com/fjn7iuq3bsK0mnFJyqFu Ever struggle to understand how separation works in HPLC You're not alone. In this video Dr. Lee Polite breaks down the chemistry behind chromatography using the worlds most approachable demo: grape Kool-Aid. Timestamps 00:00 Intro 00:37 How does HPLC chromatography work 00:52 Shopping mall analogy 05:38 About the Kool Aid experiment 07:47 Polarity explained 09:13 Start of experiment Learn the key concepts that make liquid chromatography work including:"
YouTube Link 2025-07-07T11:53Z [----] followers, [----] engagements

"Gas Chromatography Demystified - Understanding How A GC Works 👉 Want on-demand training on GC and HPLC Check out Axion Lite our free online platform with videos tips and expert training: https://courses.axionlabs.com/course/axion-lite/#/sales Feeling intimidated by Gas Chromatography (GC) 🤔 Think it's too complex with all those buttons gases and parts This video breaks down GC operation into easy-to-understand steps Timestamps 00:00 Video overview 01:12 Understanding gases in GC helium cylinders 04:04 Understanding gas generators nitrogen hydrogen zero air 09:00 Understanding how gases flow"
YouTube Link 2025-04-17T19:37Z [----] followers, 12.7K engagements

"LC/GC Bootcamp - Master HPLC & GC in one week at Axion Training Institute's Check out our in person and virtual courses: https://axionlabs.com/lc-gc-bootcamp/ https://axionlabs.com/advanced-hplc/ https://axionlabs.com/advanced-gc/ https://axionlabs.com/lc-gc-bootcamp-virtual-course/ Learn HPLC and GC at Axion Training Institutes 5-day LC/GC Bootcamp. Get hands-on applied training in our chromatography training center in Chicago. Get course dates and more information at https://axionlabs.com/lc-gc-bootcamp/"
YouTube Link 2023-10-20T20:45Z [----] followers, [----] engagements

"How to install an HPLC column"
YouTube Link 2022-10-05T14:51Z [----] followers, [----] engagements

"Top [--] tips for HPLC method development Check out our in person and virtual courses: https://axionlabs.com/lc-gc-bootcamp-virtual-course/ https://axionlabs.com/lc-gc-bootcamp/ https://axionlabs.com/advanced-hplc/ https://axionlabs.com/advanced-gc/ https://axionlabs.com/lc-gc-bootcamp-virtual-course/ Develop HPLC methods with two tips from Axion Labs: [--]. Don't reinvent the wheel - check out working methods in an HPLC column catalog. [--]. Perform a scouting run. Check out https://axionlabs.com/ for upcoming course dates"
YouTube Link 2021-04-05T12:37Z [----] followers, 13.1K engagements

"Tour of Axion Labs I've been teaching chromatography for over [--] years and I've learned that the learning environment is just as important as what I know and can teach. That's why I am so proud of Axion Labs. I've been working for over [--] years to make it the best learning environment so that you can master HPLC and GC. Check out our 5-day hands-on LC/GC Bootcamp schedule for [----] here: https://axionlabs.com/lc-gc-bootcamp/ 0:00 Chromatography Happy Place - the HPLC's and GC's dedicated to training 0:40 Where the magic happens - aha moments galore in the classroom 0:59 What you need to bring"
YouTube Link 2022-11-03T17:52Z [----] followers, [---] engagements

"Welcome to Axion Labs & Training Institute What can you expect from Axion's HPLC & GC training courses Rookie to Pro Experienced to Expert After completing Axion's HPLC & GC training courses you will have advanced HPLC and GC skills no matter your starting point. Learn more at https://axionlabs.com/ #chromatography #analyticalchemistry #scienceexplained #chemistry #chemistrytips #hplc #hplccolumns #labtechniques"
YouTube Link 2022-08-10T11:59Z [----] followers, [----] engagements

"How to convert your gas chromatograph from helium to hydrogen - Axion Labs Webinar Check out our first online virtual course: https://axionlabs.com/lc-gc-bootcamp-virtual-course/ Chapters 0:00 Why hydrogen for my GC 2:54 Axion Training Institute 6:06 What is gas chromatography 10:27 GC Theory basics 17:04 GC Instrumentation overview 18:41 Switch to hydrogen in one step 22:21 Example chromatograms 23:31 Safety considerations 26:06 A real GC run with helium 28:31 Switching to hydrogen 31:53 Tour of Axion Labs 34:38 A real GC run with hydrogen 37:33 Method optimization with hydrogen 41:40"
YouTube Link 2021-04-13T17:16Z [----] followers, [----] engagements

"Agilent Lab Advisor https://www.agilent.com/en/product/liquid-chromatography/hplc-ce-software/instrument-control-software/lab-advisor-software"
YouTube Link 2022-09-01T15:04Z [----] followers, [----] engagements

"How to choose the right HPLC column - AND - favorite HPLC columns from Agilent Waters & Phenomenex How do I choose the right HPLC column So Step [--] - look in a column catalog. Its gonna give you a guaranteed result. OK let's say that you don't have a column catalog. Or lets say that your chemical isnt in one. Lets say that you are sitting there saying Well Lee if I knew all of that I wouldnt be asking you for advice. So that is a valid point. Let me take you to the next step. In HPLC we have dozens of columns out there but there is one column that dominates. If you look at all the column sales"
YouTube Link 2021-08-27T19:22Z [----] followers, [----] engagements

"Air bubble in the pump. The simple fix #chromatography #hplc @axionlabs"
YouTube Link 2023-03-28T13:13Z [----] followers, 16K engagements

"How are HPLC and GC used in the pharmaceutical industry Read the blog here: https://axionlabs.com/chromatography-training/chromatography-applications-in-pharmaceuticals/ The pharmaceutical industry is huge in chromatography because in that industry they must by law analyze their raw materials to prove that theres nothing bad in it. Pharmaceutical companies are always looking for top professionals with valuable skills. Axion offers hands-on HPLC (High-performance liquid chromatography or high-pressure liquid chromatography) and GC (gas chromatography) training courses. Gain advanced HPLC & GC"
YouTube Link 2021-04-19T13:36Z [----] followers, 11.4K engagements

"It doesn't matter which brand GC or HPLC you use. Agilent Waters Shimadzu PerkinElmer Does it matter which one I use. NO I'm a non-denominational instructor. In other words a GC is a GC. Read the blog here: https://axionlabs.com/chromatography-training/does-it-matter-which-hplc-or-gc-brand-i-use/ 🔗 Follow Us: Website: https://axionlabs.com/ Facebook: https://www.facebook.com/axionlabs LinkedIn: https://www.linkedin.com/company/axion-labs/ #analyticalchemistry #hplc #Chromatography #LabTechniques #ScientificResearch #Chemistry #labtips Axion offers hands-on HPLC (High-performance liquid"
YouTube Link 2022-12-05T20:50Z [----] followers, [---] engagements

"Why is the GC beeping Why is the gas chromatograph beeping How do I Fix it Learn how to troubleshoot a beeping Gas Chromatograph (GC) Discover why the instrument might be beeping and how to check its status for error messages like "no hydrogen gas" or "issue getting the flame lit". See how adjusting the pressure can make your GC "happy" and stop the beeping 📺 Related Video: GC Operations Explained Full System Breakdown https://youtu.be/LaYPRxOuu54 🎓 Want to go deeper Get a free Axion Lite membership: https://courses.axionlabs.com/course/axion-lite#/salesticketId=3283437000000185030 🌐 Learn"
YouTube Link 2025-06-05T19:45Z [----] followers, [---] engagements

"HPLC Diode array parameters and tools - REFERENCE WAVELENGTH PEAK PURITY and THRESHOLD 💡 Should the Spectra Range Include the Reference Wavelength Let's break it down In this video we explore reference wavelengths in chromatography why they matter and whether they should be included in spectral data. Plus we dive into peak purity analysis and how to detect coelution using ChemStation. 🚀 🔹 Topics Covered: ✔ What happens when reference wavelength is activated ✔ How to prevent negative peaks ✔ Understanding diode array detector (DAD) signal processing ✔ Peak purity analysis and how to detect"
YouTube Link 2025-03-18T20:06Z [----] followers, [---] engagements

"Chromatography Flow Rates Explained: HPLC LC-MS & GC Whats the Best Flow Rate for starting a new chromatography method 🚀 Starting a new chromatography method and unsure about the ideal flow rate In this video we break down HPLC LC-MS and GC flow rates so you can optimize your method development with confidence. Heres what youll learn: 0:06 HPLC Flow Rates Why [---] mL/min is a great starting point for a [---] mm ID column 0:46 LC-MS Adjustments How to scale down your flow rate for [---] mm ID columns 1:05 GC Flow Rates Why I prefer to use linear velocity (25 cm/sec) Read the blog here:"
YouTube Link 2025-01-21T16:21Z [----] followers, [---] engagements

"How to develop a method for GC - top [--] tips How do you develop a method for GC Ill give you three quick trips for GC method development. 00:11 Intro and Analogy 00:58 #1 Look for a method in a column catalog 01:28 #2 Choose a good standard column 02:15 #3 Try all the temperatures in the universe 📌 Want more chromatography tips Join our free community to receive HPLC and GC training from an expert. https://courses.axionlabs.com/course/axion-lite/#/home GC method development sounds daunting But the reality is that it is a lot simpler than it sounds. Trick #1: Look in a column catalog. I know"
YouTube Link 2021-07-29T21:16Z [----] followers, [----] engagements

"What is void volume void time t0 and solvent front 🔗 Follow Us: Website: https://axionlabs.com/ Facebook: https://www.facebook.com/axionlabs LinkedIn: https://www.linkedin.com/company/axion-labs/ #analyticalchemistry #hplc #Chromatography #LabTechniques #ScientificResearch #Chemistry #labtips Axion offers hands-on HPLC (High-performance liquid chromatography or high-pressure liquid chromatography) and GC (gas chromatography) training courses. Gain advanced HPLC & GC skills with our [--] day hands-on LC/GC bootcamp. If you can't make it to Chicago choose one of our online courses. After taking"
YouTube Link 2024-02-20T15:03Z [----] followers, [----] engagements

"Can acetonitrile cause amine impurities to form deposits in HPLC pump Have you ever wondered if acetonitrile in HPLC can cause amine impurities or lead to sticky deposits in your pump In this video we explore whether acetonitrile polymerization is a real issue and how to prevent potential HPLC check valve problems. 🔬 Key Topics Covered: ✅ Does acetonitrile contain amine impurities ✅ Can acetonitrile deposits damage HPLC pumps ✅ Are certain HPLC systems more affected ✅ Best solvents to flush your HPLC system. ✅ The real issue: Buffer precipitation vs. polymerization. 💡 Pro Tips for HPLC"
YouTube Link 2025-02-03T17:14Z [----] followers, [---] engagements

"HPLC Tubing & Fittings 🔬 Welcome to our educational series on High-Performance Liquid Chromatography (HPLC). In this episode we delve into the intricacies of HPLC tubing and fittings essential components in liquid chromatography systems. What's Inside: Stainless Steel vs. PEEK Tubing: Understand the strengths and limitations of stainless steel and PEEK (polyetheretherketone) tubing in HPLC applications. Discover why stainless steel known for its high pressure handling is challenging to cut and why PEEK offers more flexibility in cutting. Fittings Types & Techniques: Learn about the"
YouTube Link 2023-09-12T16:22Z [----] followers, [----] engagements

"3 Essential Gas Generators for Gas Chromatography Explained: Hydrogen Air & Nitrogen Generators 🚨 Upgrade Your GC Lab: Why Smart GC Labs Use These [--] Gas Generators Looking to ditch your gas cylinders and streamline your gas chromatography (GC) workflow In this video we break down the [--] essential gas generators every GC lab should consider: ✅ Hydrogen Generator For carrier gas & FID fuel ✅ Zero Air Generator For hydrocarbon-free oxidant gas ✅ Nitrogen Generator For make-up gas & detector efficiency 🔬 We explain how each generator improves lab safety efficiency and cost savings and why so"
YouTube Link 2025-05-17T03:15Z [----] followers, [---] engagements

"black friday sale (720 x [---] px).mp4 Be ready for [----] with serious HPLC and GC skills. Get 25% off the Dec. 9-13 session of the hands-on LC/GC Bootcamp at Axion Labs. https://zurl.co/cadj"
YouTube Link 2024-11-25T17:04Z [----] followers, [---] engagements

"In gas chromatography what is column flow purge flow split ratio 💡 Understanding Column Flow Purge Flow and Split Ratio in Gas Chromatography (GC) 💡 In this video we break down the key concepts of column flow purge flow and split ratio in GC: ✅ Column Flow The carrier gas moving through the column ✅ Purge Flow A small stream (3 mL/min) that removes contaminants from the septum ✅ Split Flow The portion of the sample that is vented out instead of entering the column ✅ Split Ratio The ratio of split flow to column flow (e.g. 100:1 means 1% of the sample enters the column) Read the blog here:"
YouTube Link 2025-02-17T16:25Z [----] followers, [----] engagements

"HPLC Demystified Part [--] How to Maintain and Troubleshoot Your HPLC Like a Pro 🎁Download our HPLC Maintenance & Troubleshooting Checklist https://zfrmz.com/fjn7iuq3bsK0mnFJyqFu 🔧 HPLC Demystified Part 3: How to Maintain and Troubleshoot Your HPLC Like a Pro Chromatography giving you headaches Part [--] of the HPLC Demystified series is your lab-side guide to troubleshooting and routine maintenance explained clearly by Dr. Lee Polite. Whether you're dealing with air bubbles leaky seals noisy baselines or a worn-out rotor seal this video walks you through the why the how and the fix. 00:00 Intro"
YouTube Link 2025-08-28T11:43Z [----] followers, [----] engagements

"GC Sample Prep Essentials: Nailing Solvents Dilution & Injection 👉 Want to learn more about running the instrument Watch our video on GC Operations: https://youtu.be/LaYPRxOuu54 👉 More in-depth training and free videos at Axon Lite: https://courses.axionlabs.com/course/axion-lite/#/sales Struggling to get consistent reliable results from your Gas Chromatograph Proper sample preparation is the critical first step and in this video we break down the absolute essentials for chemists. Learn why sample volatility is non-negotiable in GC analysis and how anything non-volatile gets left behind in"
YouTube Link 2025-04-18T19:00Z [----] followers, [---] engagements

"Clean a reverse phase HPLC Column in [--] steps. Cleaning a reverse-phase HPLC column doesnt have to be complicated. In this step-by-step guide Dr. Lee Polite explains how to flush out salts buffers and sticky non-polar residues using simple solvent changes. Youll learn exactly when to use water-rich mobile phase when to switch to 100% organic and how to keep your columns running longer. Prefer to read: https://axionlabs.com/chromatography-training/clean-reverse-phase-hplc-column/ 👉 Related Videos: How to Clean a C18 Column extra details on cleaning a dirty or clogged frit"
YouTube Link 2025-09-10T22:19Z [----] followers, [----] engagements

"HPLC problems with very polar molecules. 🔬 Welcome to the world of HPLC columns specifically focusing on the 'Polar Embedded Phase' technology 🔍 In this video we unravel the intricacies of these highly efficient columns. Read the blog here: https://axionlabs.com/chromatography-training/hplc-problems-with-very-polar-molecules/ 📌 Don't forget to like subscribe and hit the bell icon for notifications on our upcoming videos Share your thoughts and questions in the comments section below. 🔗 #HPLCColumns #AnalyticalChemistry #PolarEmbeddedPhases #Chromatography #ScienceExplained"
YouTube Link 2024-01-08T16:37Z [----] followers, [---] engagements

"Monomeric vs Polymeric C18 Columns Monomeric vs. Polymeric C18 Columns: Whats the Difference 🤔💡 When it comes to chromatography the choice between monomeric and polymeric C18 columns can have a major impact on your separations. But what exactly sets them apart In this video we break down: ✅ The fundamental differences between monomeric and polymeric C18 columns ✅ How shape selectivity in polymeric C18s allows for unique separations ✅ Real-world applications including polynuclear aromatic hydrocarbons (PAHs) and complex mixtures ✅ When to use monomeric vs. polymeric C18 columns for optimal"
YouTube Link 2024-03-05T17:46Z [----] followers, [---] engagements

"HPLC Method Tips for Aromatic Compounds in Water Based Gels: Why C18 Still Works Learn how to analyze aromatic compounds in water-based gels using HPLC and why a C18 column is still one of the most reliable choices. In this clip Dr. Lee Polite breaks down how water interacts with a C18 stationary phase why gel matrices arent a problem in reverse-phase chromatography and how to set up a simple gradient method to get clean dependable separations. If youre working with gels creams lotions or other complex water-based materials this video will help you choose the right column solvent system and"
YouTube Link 2025-11-18T19:29Z [----] followers, [---] engagements

"HPLC Demystified Part [--] How an HPLC System Works. 🎁Download the PDF lesson guide https://zfrmz.com/fjn7iuq3bsK0mnFJyqFu Think HPLC systems are complicated Not after this. In Part [--] of the HPLC Demystified series Dr. Lee Polite opens the hood of a real instrument and walks you through each component in a way that finally makes sense. Whether youre prepping for a job troubleshooting in the lab or just want to stop feeling intimidated by your LC system this video is for you. 00:00 Intro 00:28 Mobile Phase (Solvent Bottles) 02:53 Degasser 05:44 Pro Tip About The Degasser 07:06 Pump 13:55"
YouTube Link 2025-07-16T12:49Z [----] followers, [----] engagements

"Co-Elution: How to Detect and Fix Overlapping Peaks. Co-elution is the silent killer of chromatography datatwo compounds exit the column at the same time creating one misleading peak. That means you can't identify quantify or trust your results. In this video Dr. Lee Polite explains what causes co-elution how to detect it using peak shape DAD and Mas Spec and how to fix it using the resolution equation: capacity factor selectivity and efficiency. Perfect for anyone working with HPLC or GC especially if you're troubleshooting poor resolution or planning method development. 📘 Explore hands-on"
YouTube Link 2025-07-03T14:20Z [----] followers, [----] engagements

"How to clean a C18 column 🔬 In this video guide we explore the necessary techniques for maintaining the efficiency of your C18 column in chromatography. Our expert tips help identify column issues and offer practical cleaning solutions. 🌐 Highlights of the Article: Identifying Column Issues: Learn how to detect and prevent permanent damages to your HPLC column. Dealing with Dirty or Clogged Frits: Discover the reasons behind frit clogging and a controversial yet effective cleaning method. Watch this webinar to see how we properly prepare a sample for HPLC."
YouTube Link 2023-10-24T15:30Z [----] followers, [----] engagements

"HPLC Troubleshooting Reproducibility Tips HPLC Reproducibility Problems Its NOT Your Method. Heres What to Check First. Getting different chromatograms from the same HPLC sample is one of the most frustrating problems in the lab. Different peak areas. Different heights. Sometimes even a different number of peaks. In this short video Dr. Lee Polite explains why this is not a method problem and how to quickly diagnose reproducibility issues in your HPLC system without rewriting your method. Youll learn: -Why the same vial should always produce the same chromatogram -What extra or missing peaks"
YouTube Link 2026-01-08T16:56Z [----] followers, [---] engagements

"Do I need to use an HPLC Guard Column In this video we focus on the use of guard columns. We answer these questions: What's the deal with the guard column Do I need one Is it required What's it for 🔬 What You'll Learn: The fundamental purpose of a guard column in chromatography. How to decide if a guard column is necessary for your specific application. Tips for maintaining your analytical column and extending its lifespan. Real-world insights into how guard columns can affect your results. 🔗 Useful Links: How to clean a C18 column:"
YouTube Link 2023-12-12T20:32Z [----] followers, [----] engagements

"How to print an HPLC calibration curve How to print an HPLC calibration curve Go to File then Print and then navigate over to Calibration Table and Curves. If you click that it's a beautiful printout. This question came from this video - https://youtu.be/p1qN0szuqjcsi=EpQEIfeAbVCV-EbH 📌 Want more chromatography tips Join our free community to receive HPLC and GC training from an expert. https://courses.axionlabs.com/course/axion-lite/#/home 📌 Got more HPLC questions Drop them in the comments 🔔 Subscribe for more chromatography insights #HPLC #Chromatography #LabScience #HPLCAnalysis"
YouTube Link 2025-03-11T20:45Z [----] followers, [---] engagements

"GC Troubleshooting - The most common problems you will encounter. Check out our first online virtual course: https://axionlabs.com/lc-gc-bootcamp-virtual-course/ Chapters: 0:00 What is wrong with my GC - an overview 0:30 Half splitting technique 1:17 #1 a leaky septum 2:25 #2 the liner 3:41 GC troubleshooting at Axion Labs How do I know if my septum is leaking When do I change my liner How can I learn GC troubleshooting Dr. Polite gives some quick tips to help you with your GC troubleshooting"
YouTube Link 2021-04-19T13:23Z [----] followers, [----] engagements

"What is the difference between running green stainless vs red stainless HPLC tubing at 0.3mL/min Are you running your HPLC system at [---] mL/min and wondering whether to use green or red stainless steel tubing In this video we break down the key differences between these two tubing options and explain how choosing the right one can improve efficiency and peak resolution in low-flow applications like LC-MS. 💡 Key Topics Covered: ✅ Why green tubing (0.17 mm ID) is standard for most flow rates ✅ When to switch to red tubing (0.12 mm ID) for low-flow applications ✅ How tubing diameter affects"
YouTube Link 2025-02-11T15:23Z [----] followers, [---] engagements

"Understanding The Master Resolution Equation In Chromatography Unlock the secrets to perfect peak separation in your chromatographic methods In this comprehensive guide we break down the Master Resolution Equation a fundamental concept in chromatography (liquid chromatography gas chromatography) that once understood can significantly simplify your method development and troubleshooting efforts. Stop the guesswork and start making informed decisions Learn how the three critical factors Efficiency (N) Selectivity () and Capacity Factor (k) directly impact your separation quality. We explain"
YouTube Link 2025-05-22T16:10Z [----] followers, [---] engagements

"Why Your GC Inlet Total Flow Drops During a Temperature Ramp And How to Fix It Why does your GC inlet total flow drop during an oven temperature ramp In this video Dr. Lee Polite explains how gas viscosity changes during heating why constant pressure causes flow decline and why switching to constant flow mode gives you better more stable chromatography. Watch the video GC Troubleshooting - The most common problems you will encounter. : 👉 https://www.youtube.com/watchv=WMa-oIR18S8 Learn more about GC & HPLC at Axion Labs: https://axionlabs.com #gaschromatography #gctroubleshooting"
YouTube Link 2025-12-08T16:23Z [----] followers, [---] engagements

"Superficially Porous vs Totally Porous HPLC Columns Higher Efficiency Lower Pressure Whats the difference between superficially porous (SPP) and totally porous (TPP) particles in HPLC columns In this video we explain how column particle structure affects efficiency separation and pressure requirements. Youll learn: Why smaller HPLC particles increase efficiency but demand higher pressure How superficially porous particles (core-shell) give you UPLC-like efficiency on standard HPLC systems The pros and cons of SPS vs TPS columns for real-world chromatography applications Whether youre running"
YouTube Link 2025-08-19T20:08Z [----] followers, [---] engagements

"Why My HPLC Columns Last [--] Years: The Truth About pH and Column Damage. Unlock the real secret to HPLC column longevity. Most scientists think column failure comes from dirty samples or too many injections but thats not the real culprit. In this video Dr. Lee Polite explains why pH is the single most important factor that determines whether your column lasts [--] injections or [--] years. Youll learn: Why sample pH almost never matters. The exact pH range every silica-based column loves. What happens chemically above pH [--] and below pH [--]. When it's safe to cheat at extreme pH. Which specialty"
YouTube Link 2025-11-25T19:38Z [----] followers, [---] engagements

"Demystifying GC Method Development - When to Change Your Column 👉 Get the Chromatography Demystified Mini-course with a free Axion Lite membership. https://courses.axionlabs.com/course/axion-lite/#/sales Separating xylene isomers is a challenging real-world problem in gas chromatography. In this video Dr. Lee Polite walks step by step through GC method development using a classic xylene mixture: ortho- meta- para-xylene and ethylbenzene. Youll see what happens when a standard scouting run produces co-elution why temperature and flow optimization alone are sometimes not enough and how"
YouTube Link 2026-02-10T16:10Z [----] followers, [---] engagements

"Can I run an HPLC gradient from [--] to 100% For related videos watch: Can you run 100% water with a C18 column https://youtu.be/xwMYDUYPJR0 HPLC Problems: Very polar molecules. https://youtu.be/HZV5B_p6OeM Read the blog post here https://axionlabs.com/chromatography-training/can-i-run-an-hplc-gradient-from-0-to-100/ Subscribe for more insights into chromatography techniques and applications. 🔗 Follow Us: Website: https://axionlabs.com/ Facebook: https://www.facebook.com/axionlabs LinkedIn: https://www.linkedin.com/company/axion-labs/ #analyticalchemistry #hplc #Chromatography #LabTechniques"
YouTube Link 2024-02-27T19:20Z [----] followers, [---] engagements

"What's the difference between HPLC & UPLC Let me give you the technical difference between HPLC & UPLC. UPLC first of all is a registered trademark of Waters Corporation. Other companies call their version of UPLC something like UHPLC or STM or something other than UPLC. Read the blog here: https://axionlabs.com/chromatography-training/uplc-and-hplc-whats-the-difference-video/ 🔗 Follow Us: Website: https://axionlabs.com/ Facebook: https://www.facebook.com/axionlabs LinkedIn: https://www.linkedin.com/company/axion-labs/ #Chromatography #HPLC #ScientificResearch #analyticalchemistry #chemistry"
YouTube Link 2023-10-17T16:00Z [----] followers, [----] engagements

"How does mass spec work Watch the full webinar on Axion Lite - Free HPLC and GC training. https://courses.axionlabs.com/course/axion-litepreviouspage=allcourses&isenrolled=no#/salesticketId=3283437000000185030 Subscribe for more insights into chromatography techniques and applications. 🔗 Follow Us: Website: https://axionlabs.com/ Facebook: https://www.facebook.com/axionlabs LinkedIn: https://www.linkedin.com/company/axion-labs/ #analyticalchemistry #hplc #Chromatography #LabTechniques #ScientificResearch #Chemistry #labtips Axion offers hands-on HPLC (High-performance liquid chromatography"
YouTube Link 2024-05-14T18:22Z [----] followers, [---] engagements

"Importance of Innovation in Gas Chromatography Agilent introduced their revolutionary new type of GC on 8/30/2016 at their headquarters in Little Falls Delaware: the Agilent [----] Intuvo. Six years ago Dr. Polite was one of only [--] thought leaders from around the world called in to help Agilent with the design criteria. Lee was often referred to as the voice of the customer during the design process. The goal was to make the GC easier to use while not sacrificing any of the chromatographic performance. It has been [--] years in the making and the result is pretty impressive. No more column nuts"
YouTube Link 2016-08-30T21:22Z [----] followers, [---] engagements

"12 most commonly asked HPLC and GC interview questions with answers. Get the extended version of this video plus download the Interview Prep Guide. https://axionlabs.com/hplc-gc-interview-guide/ Are you preparing for an HPLC or GC job interview Get a massive advantage with this expert guide In this video Dr. Lee Polite a seasoned chromatography expert reveals the [--] most commonly asked HPLC and GC interview questions and provides the practical insightful answers that hiring managers are truly looking for. Don't let lack of experience hold you back. Prepare smarter interview confidently and"
YouTube Link 2025-06-17T13:10Z [----] followers, [----] engagements

"Pyrolysis GC: How to Analyze Paint Plastics and Polymers with Gas Chromatography. 🔗 Resources Mentioned Watch the full GC operations video: https://youtu.be/LaYPRxOuu54si=Lmh18rfrW-g8R5Gb 🎓 Want to go deeper Visit Axion Lite for on-demand training in GC and HPLC: https://courses.axionlabs.com/course/axion-lite#/salesticketId=3283437000000185030 🔥 What Is Pyrolysis GC and Why Does It Matter In this video we break down one of the most fascinating techniques in gas chromatographyPyrolysis GC. Discover how scientists and forensic experts use this method to analyze plastics paints and polymers"
YouTube Link 2025-04-22T14:37Z [----] followers, [---] engagements

"Dwell Volume vs Dead Volume Let me make a couple of clarifications here. Instead of using the term dead volume we prefer the term extra column volume. Extra column volume indicates the volume outside of the column which we aim to minimize. Minimizing extra column volume is crucial for all our LCs as it affects our efficiency and the skinniness of our peaks. Read the blog article here https://axionlabs.com/chromatography-training/dwell-volume-vs-dead-volume-whats-the-difference/ Subscribe for more insights into chromatography techniques and applications. 🔗 Follow Us: Website:"
YouTube Link 2024-03-11T16:42Z [----] followers, [----] engagements

"Demystifying GC Method Development 👉 Get the Chromatography Demystified Mini course with a free Axion Lite membership. https://courses.axionlabs.com/course/axion-lite/#/sales In this in-depth session Dr. Lee Polite walks shows how to develop and optimize a gas chromatography (GC) method from the ground up. He starts with a simple universal scouting run using a standard non-polar column (DB-5 type) and a wide temperature program. From there Dr. Polite demonstrates how to systematically optimize for speed and efficiency cutting run times dramatically by adjusting linear velocity (flow rate)"
YouTube Link 2026-01-27T20:36Z [----] followers, [---] engagements

"Understand and Fix Negative Peaks - Refractive Index (RI) Detectors 🔬 This video dives into the mystery of negative peaks specifically with refractive index (RI) detectors Learn why they happen (it's not an error) and how to easily integrate them using your software. We also touch on unexpected negative peaks in UV detection and what they might mean. Read the blog article: https://axionlabs.com/chromatography-training/negative-peaks-in-hplc-refractive-index-and-uv-detectors/ 🚀 Stop scratching your head and master your HPLC data 📌 Want more chromatography tips Join our free community to"
YouTube Link 2025-05-10T14:15Z [----] followers, [----] engagements

"When to use a gradient in HPLC How do you know when you should use an gradient elution instead of isocratic elution In this exploration of gradients in chromatography we unpack the pivotal question: when is a gradient necessary The heart of the matter lies in the hydrophobic similarities of the molecules being analyzed. If they share similar hydrophobicities a gradient might not be needed as indicated by a scouting run where peaks emerge closely together. However for a diverse range of hydrophobicities or unknown samples gradients shine by offering versatility and the invaluable ability to"
YouTube Link 2024-03-19T15:24Z [----] followers, [----] engagements

"Will highly acidic or basic samples affect an HPLC C18 column Should you worry about the pH of a sample damaging the column What happens when your sample isnt in the same pH as the mobile phase I break it all down in simple terms So this is in response to a video I did about the use of guard columns. https://axionlabs.com/chromatography-training/understanding-hplc-guard-columns-necessity-and-best-practices/ The general rule in HPLC is that we always want to dissolve the sample in the mobile phasethat is the ideal situation. If the sample solvent is the same as the mobile phase it will just"
YouTube Link 2025-03-04T19:29Z [----] followers, [---] engagements

"How to test potency of THC GC or HPLC 🧪 How to Test THC Potency GC vs. HPLC Are you wondering which instrument is best for testing THC potency in cannabisGas Chromatography (GC) or High-Performance Liquid Chromatography (HPLC) In this video we break down the key differences. 00:00 🔬 How THC potency is measured 01:14 📊 HPLC vs. GC Key differences 02:00 💡 Use HPLC for differentiating THC & THCA 02:05 🔥 GC is best for total THC measurement Read the blog here: https://axionlabs.com/chromatography-training/how-to-test-thc-potency-gc-or-hplc/ Want more tips about sample prep Watch the webinar"
YouTube Link 2025-02-25T17:31Z [----] followers, [----] engagements

"HPLC Columns Explained Understanding Carbon Load and Surface Area for Better Separations Join our free community to get on-demand courses: https://courses.axionlabs.com/course/axion-lite/#/sales Why does one C18 column behave differently from another The answer often comes down to two overlooked details: carbon load and surface area. In this video Dr. Lee Polite breaks down: How carbon load is measured in HPLC columns Typical carbon load ranges (7% to 30%) and what they mean Why higher or lower carbon load changes retention and capacity How surface area (100300 m/g) impacts column performance"
YouTube Link 2025-09-03T12:22Z [----] followers, [---] engagements

"How to fix "GC-MS vial out of range" error -🔧 GC-MS Error: Vial Out of Range Quick Fix Explained Running into a "vial out of range" error on your Agilent GC or GCMS system Dont worryits a common mistake with an easy fix. In this video we walk through what causes this error and how to solve it especially if you're using an Agilent [----] autosampler. 👉 Want more FREE training on GC and HPLC Check out Axion Lite our free online platform with videos tips and expert training: https://courses.axionlabs.com/course/axion-lite/#/sales 📌 Topics Covered: -Mass Spec [--] part series -HPLC Method"
YouTube Link 2025-04-02T16:01Z [----] followers, [---] engagements

"HPLC: Analyzing a New Sample - Step By Step Get the links for each individual part of the series and [--] bonus videos: Building a method in ChemStation and HPLC method optimization. https://axionlabs.com/chromatography-training/hplc-basics-video-analyzing-a-new-sample/ Join our free community to receive all of our webinars. https://courses.axionlabs.com/course/axion-lite/#/home Learning the basics of HPLC and some principle of analytical chemistry will help you confidently analyze your own samples from scratch. In this labinar series we show you step-by-step how to think about analyzing an"
YouTube Link 2024-11-25T23:45Z [----] followers, [---] engagements

"How to test a LC column In this video we dive deep into the best ways to test an LC column's performance to ensure optimal results. We'll explore different methods for testing columns including using specific probes like dimethyl aniline and quinizarin to check for acidity and metal content. However we emphasize a more practical approach: using your daily standard samples to evaluate column efficiency and peak shapes. Learn how to measure column efficiency understand the significance of your column's certificate of performance and know when it's time to replace a column. Discover expert tips"
YouTube Link 2023-11-13T16:30Z [----] followers, [----] engagements

"What's unique about Axion courses See what an Axion course is like. Subscribe for more insights into chromatography techniques and applications. 🔗 Follow Us: Website: https://axionlabs.com/ Facebook: https://www.facebook.com/axionlabs LinkedIn: https://www.linkedin.com/company/axion-labs/ #analyticalchemistry #hplc #Chromatography #LabTechniques #ScientificResearch #Chemistry #labtips Axion offers hands-on HPLC (High-performance liquid chromatography or high-pressure liquid chromatography) and GC (gas chromatography) training courses. Gain advanced HPLC & GC skills with our [--] day hands-on"
YouTube Link 2024-04-01T19:57Z [----] followers, [----] engagements

"Can you run 100% water with a C18 column 🔬 Mastering Your C18 Column: Essential Tips for Chromatographers 🔬 👉 What's Inside: The Water Dilemma: Can you run your C18 column with 100% water We unravel this common question and explain why it's not recommended. Discover the impact of water on the hydrophobic nature of C18 and how it leads to phase collapse reducing chromatography efficiency. Recovery Tactics: Accidentally used too much water No worries We show you how to bring your column back to life with 100% acetonitrile or methanol. Optimal Organic Content: Learn the importance of"
YouTube Link 2024-01-25T15:17Z [----] followers, [----] engagements

"Everything you need to know about HPLC flow rates HPLC flow rates very depending on the column diameter. In this video we talk about columns ranging from [---] to [--] millimeter ID. Read the full post here: https://axionlabs.com/chromatography-training/hplc-flow-rates-everything-you-need-to-know/ Subscribe for more insights into chromatography techniques and applications. 🔗 Follow Us: Website: https://axionlabs.com/ Facebook: https://www.facebook.com/axionlabs LinkedIn: https://www.linkedin.com/company/axion-labs/ #analyticalchemistry #hplc #Chromatography #LabTechniques #ScientificResearch"
YouTube Link 2024-03-29T18:39Z [----] followers, [----] engagements

"Best solvent to dissolve HPLC samples 🔬 Welcome to our latest educational video on chromatography In this comprehensive guide we delve into the critical aspect of selecting the perfect sample solvent for effective chromatography. Whether you're a seasoned chemist or a student just starting out this video offers valuable insights for everyone. 🌟 What's Inside: Impact of Sample Solvent on Chromatography: We start by exploring how the choice of solvent affects the overall chromatography process emphasizing the importance of this decision. Best Practices for Solvent Selection: Next we provide"
YouTube Link 2023-11-21T17:06Z [----] followers, [----] engagements

"HPLC Method Development is like baking a cake. #hplc #analyticalchemistry"
YouTube Link 2023-06-19T17:50Z [----] followers, [----] engagements

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